Extended data - Increased <i>in vivo</i> transduction of AAV-9 cargo in Alport podocytes
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Extended data for manuscript - <b>Increased </b><b><i>in vivo</i></b><b> transduction of AAV-9 cargo in Alport podocytes</b>This project contains the following extended data:<b>Extended data S. Method</b> - Picrosirius staining and quantification pipeline<b>Extended data S. Figure 1</b>- <b>AAV9-GFP-WPRE reporter plasmid</b>. Circular map of the AAV9-GFP reporter construct with the regulatory elements. (Aldevron plasmid # 5069-10; <i>https://www.aldevron.com/catalog-products/viral-vector-plasmids/pald-aav</i>)<b>Extended data S. Figure 2</b> - <b>Image panel of isolated mouse glomeruli transduced with AAV9-GFP.</b> Images of 10 wild type glomeruli isolated from 8-week-old mice (control, no AAV9-GFP); 12 wild type and <i>Col4a5 KO</i> glomeruli transduced with AAV9-GFP. Scale bar= 25 µm.<b>Extended data S. Figure 3</b> - <b>Representative images of liver cross-sections from 12-week-old mice injected with AAV9-GFP.</b> A) Representative images of liver cross-sections from 12-week-old mice injected with 1.5 x 10<sup>14</sup> vg/kg (high dose) of AAV9-GFP. Quantification of GFP intensity in the liver. Scale bar= 1000 µm. Zoomed in images, scale bar= 100 µm. B) Schematic representation and quantification of liver stained with picrosirius red.
