Table S1 - Generation of Immortal Cell Lines from the Adult Pituitary: Role of cAMP on Differentiation of SOX2-Expressing Progenitor Cells to Mature Gonadotropes

Primer sequences used for RT-PCR screening. All RNA samples were DNase treated and then amplified using a one-step RT-PCR Kit as per manufacturer's instructions. PCR was conducted according to the following: 95°C for 30 s, 60°C for 30s, and 72°C for 1min (40 cycles). Annealing temperature was altered according to the corresponding primer requirements. A total of 200 ng of RNA template from each cell line was used for each reaction. All PCR-amplified products were visualized on 2% agarose gels containing ethidium bromide (final concentration of 0.05 mg/mL), under ultraviolet light. All primers were designed using mouse mRNA sequences and were made to cross at least one intron. All PCR fragments were sequenced to confirm identity. (PDF)