Characteristics and analyses of human gut samples sorted with increasing patient age.

Diagnosis: ac, adenocarcinoma; ap, apendicitis; at, atresia; d, diverticulitis; IBD, inflammatory bowel disease; NEC, necrotizing enterocolitis; p, polyps.Analyses (see ‘Methods’):IHC, immunohistochemistry (PGP9.5+ ganglia size in μm2/muscle thickness in μm); ISH, in situ hybridisation (human Alu+ cells per slice); FISH, fluorescence ISH (ToTelVysion DNA probe mixture); M, brightfield microscopy (sphere diameter in μm); NOS, diaphorase-NOS staining (% NOS+ of Fast Red plexus cells); OB, organ bath (% EFS of ACh contraction); qPCR, quantitative polymerase chain reaction (normalized 2-ΔΔct values of p75/Ret/TPM); nd = not determined; tp = technical problems (data excluded).