Dual-species microarray for assessing gene expression in stromal microenvironment, cancer cells and their interactions (v1 array)

Design a human-mouse dual-species microarray to provide a technology for investigation of cancer-stromal interaction in xenograft model. Reference RNA from human and mouse were labeled using Cy5, while the mixture of human and mouse RNA were labeled using Cy3. Samples were hybridized on customized-commercial array with Agilent probes and user-designed species-specific probes (Human-mouse dual-species array HomoMusArray v1).