Astrocyte developmental transcriptome from mouse visual cortex

Analysis of astrocyte gene expression across postnatal development in the mouse visual cortex, spanning the time of synaptogenesis and synapse maturation. Profile of astrocyte-enriched mRNA from the mouse visual cortex at 4 different developmental (P7,P14,P28,P120) timepoints from GFAP-Ribotag mice (ribosomal pulldown), with 3-5 biological replicates and 1 input (non-pulldown) sample per time point本研究针对小鼠视觉皮层出生后发育阶段的星形胶质细胞（astrocyte）基因表达展开分析，覆盖突触发生与突触成熟关键时期。实验通过核糖体下拉（ribosomal pulldown）技术，从GFAP-核糖体标签（GFAP-Ribotag）小鼠的视觉皮层中，获取了4个发育时间点（P7、P14、P28、P120）的星形胶质细胞富集mRNA谱；每个时间点设置3-5个生物学重复样本及1个输入（未下拉）对照样本。