Pre-catalytic quaternary complex of DNA Polymerase Lambda with bound complementary DSB substrate and incoming dUMPNPP

Pre-catalytic quaternary complex of DNA Polymerase Lambda with bound complementary DSB substrate and incoming dUMPNPP Descriptor: 1,2-ETHANEDIOL, 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... Authors: Kaminski, A.M, Bebenek, K, Pedersen, L.C, Kunkel, T.A. Deposit date: 2021-03-10 Release date: 2022-03-16 Last modified: 2024-11-20 Method: X-RAY DIFFRACTION (1.8 Å) Cite: Analysis of diverse double-strand break synapsis with Pol lambda reveals basis for unique substrate specificity in nonhomologous end-joining. Nat Commun, 13, 2022

结合互补双链断裂（double-strand break, DSB）底物与待掺入的dUMPNPP的DNA聚合酶λ（DNA Polymerase Lambda）催化前四元复合物。组成信息：1,2-乙二醇、2'-脱氧尿苷5'-α,β-亚氨基三磷酸、2-氨基-2-羟甲基-1,3-丙二醇……作者：A.M. 卡明斯基、K. 贝贝内克、L.C. 佩德森、T.A. 昆克尔。存档日期：2021-03-10。发布日期：2022-03-16。最后更新日期：2024-11-20。实验方法：X射线衍射（分辨率1.8 Å）。引用文献：《通过聚合酶λ分析多样双链断裂突触过程揭示非同源末端连接的独特底物特异性基础》，《自然-通讯》（Nat Commun），第13卷，2022年。