Rodent Research-1 (RR1) NASA Validation Flight: Mouse adrenal gland transcriptomic, proteomic, and epigenomic data

NASA's Rodent Research (RR) project is playing a critical role in advancing biomedical research on the physiological effects of space environments. Due to the limited resources for conducting biological experiments aboard the International Space Station (ISS), it is imperative to use crew time efficiently while maximizing high-quality science return. NASA's GeneLab project has as its primary objectives to 1) further increase the value of these experiments using a multi-omics, systems biology-based approach, and 2) disseminate these data without restrictions to the scientific community. The current investigation assessed viability of RNA, DNA, and protein extracted from archived RR-1 tissue samples for epigenomic, transcriptomic, and proteomic assays. During the first RR spaceflight experiment, a variety of tissue types were harvested from subjects, snap-frozen or RNAlater-preserved, and then stored at least a year at -80C after return to Earth. They were then prioritized for this investigation based on likelihood of significant scientific value for spaceflight research. All tissues were made available to GeneLab through the bio-specimen sharing program managed by the Ames Life Science Data Archive and included mouse adrenal glands, quadriceps, gastrocnemius, tibialis anterior, extensor digitorum longus, soleus, eye, and kidney. We report here protocols for and results of these tissue extractions, and thus, the feasibility and value of these kinds of omics analyses. In addition to providing additional opportunities for investigation of spaceflight effects on the mouse transcriptome and proteome in new kinds of tissues, our results may also be of value to program managers for the prioritization of ISS crew time for rodent research activities.

美国国家航空航天局（NASA）啮齿动物研究（Rodent Research, RR）项目正为推进太空环境生理效应相关生物医学研究发挥关键作用。由于在国际空间站（International Space Station, ISS）开展生物学实验的资源有限，高效利用航天员工作时间并最大化高质量科学产出至关重要。美国国家航空航天局GeneLab项目的核心目标有二：其一，通过多组学（multi-omics）、基于系统生物学（systems biology）的方法进一步提升此类实验的科研价值；其二，无限制地向科学界公开此类实验数据。本项研究针对存档的RR-1啮齿动物组织样本所提取的RNA、DNA与蛋白质，开展了表观基因组、转录组以及蛋白质组检测的活性评估。在首次RR太空飞行实验期间，科研人员从实验动物体内采集了多种组织样本，经快速冷冻或RNAlater保存后，在返回地球后于-80℃环境下至少存储了一年。本项研究基于这些样本在太空飞行研究中的潜在重大科研价值，对其进行了优先级排序。所有组织样本均通过由埃姆斯生命科学数据档案馆管理的生物样本共享项目提供给GeneLab，样本涵盖小鼠肾上腺、股四头肌、腓肠肌、胫骨前肌、趾长伸肌、比目鱼肌、眼部组织以及肾脏组织。本文报道了此类组织提取的实验流程与结果，进而论证了此类组学分析的可行性与科研价值。除了为探究太空环境对小鼠新型组织中转录组与蛋白质组的影响提供新的研究契机外，本研究结果还可为项目管理者合理分配国际空间站航天员用于啮齿动物研究的工作时间优先级提供参考依据。

搜集汇总

背景与挑战

背景概述

该数据集是NASA啮齿动物研究-1验证飞行项目的一部分，专注于小鼠肾上腺组织的多组学分析，包括转录组学、蛋白质组学和表观基因组学数据。数据集包含21个样本，来自16周龄雌性C57BL/6J小鼠，分为太空飞行组和地面对照组，旨在评估太空环境对生物体的生理影响。数据通过RNA测序、质谱分析和全基因组亚硫酸氢盐测序等技术生成，总大小约为921.65 GB，为研究太空飞行响应提供了重要的生物医学资源。