Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in a closed ternary complex with the artificial base pair dNaM-d5SICSTP

Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in a closed ternary complex with the artificial base pair dNaM-d5SICSTP Descriptor: (5'-D(*AP*AP*AP*(BMN)P*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3'), (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3'), 2-{2-deoxy-5-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-erythro-pentofuranosyl}-6-methylisoquinoline-1(2H)-thione, ... Authors: Betz, K, Diederichs, K, Marx, A. Deposit date: 2011-07-12 Release date: 2012-06-06 Last modified: 2023-09-13 Method: X-RAY DIFFRACTION (2.1 Å) Cite: KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry. Nat.Chem.Biol., 8, 2012水生嗜热菌（Thermus Aquaticus）来源的DNA聚合酶I大片段与人工碱基对dNaM-d5SICSTP形成闭合三元复合物的晶体结构。描述：(5'-D(*AP*AP*AP*(BMN)P*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3')、(5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3')、2-{2-脱氧-5-O-[(R)-羟基{[(R)-羟基(膦酰氧基)磷酰基]氧基}磷酰基]-β-D-赤式呋喃戊糖基}-6-甲基异喹啉-1(2H)-硫酮……作者：贝茨（Betz, K）、迪德里希斯（Diederichs, K）、马克思（Marx, A）。沉积日期：2011-07-12；发布日期：2012-06-06；最后修改日期：2023-09-13。实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率2.1埃（Å）。引用文献：《KlenTaq聚合酶通过诱导沃森-克里克（Watson-Crick）几何构象复制人工碱基对》，《自然·化学生物学》（Nat.Chem.Biol.），第8卷，2012年。