aThe ΔospC/FL/1, ΔospC/FL/2, ΔospC/ΔNt5/1 and ΔospC/ΔNt5/2 spirochetes were grown to late-log phase (108 cells per ml) and 10-fold serially diluted with BSK-H medium. Approximately 100 µl of bacterial
aMouse infection was determined 3 weeks post inoculation by serological response to B. burgdorferi proteins and reisolation of spirochetes from ear, bladder and joint tissues. bThe ID50 was calculated
Groups of five BALB/c mice were inoculated with the clone ΔdbpAB/dbpAB/1, ΔdbpAB/dbpAB/2, ΔdbpAB/dbpA'/1, ΔdbpAB/dbpA'/2, ΔdbpAB/dbpB'/1 or ΔdbpAB/dbpB'/2 and sacrificed 4 months later. One animal tha
Various genotypes of Borrelia burgdorferi sensu stricto have been previously identified among a large collection of isolates cultured from patients with Lyme disease in the United States. Furthermore,