Gene expression profiling was performed of Pax5 wild type bone marrow subsets from common lymphoid progenitors through to Hardy stage F cells. These cells were obtained by flow sorting of bone marrow.
GATA2, an essential transcription factor in fetal and adult hematopoiesis, is frequently found to be mutated in hematopoietic malignancies. However, the function of mutated GATA2 in hematopoiesis and
Intragenic PAX5 amplification in <1%of B-cell precursor acute lymphoblastic leukaemia. Affymetrix SNP 6.0 array analysis was undertaken in patients who showed amplication of PAX5 by MLPA testing usi
aProbe (r) = Reverse TaqMan MGB probe; PML primer 1 for bcr1, bcr2 and bcr3; PML primer 5 for bcr1 and bcr2; PML primer 6 for bcr3Primers and probes for 3-plex RT-qPCR of PML-RARa and ABL.
