RNA-seq analysis on primary tumor and liver metastatic lesion of CRC

To compare the expression profiles of colorectal cancers and liver metastatic lesion, 4 primary tumors and 3 liver metastases from CRC patients were subject to RNA-seq analysis Overall design: RNA of 4 primary tumors and 3 liver metastases from CRC patients were transcribed to cDNA with random primers, then the cDNA was ligated to Illumina sequencing adaptors and sequenced on the Illumina HiSeq2500.