RNA-seq analysis for DMSO- or RSL3-treated H1299 cells

Ferroptosis suppresses tumor growth. Finding out potent pro-ferroptosis regulators via new approaches is extremely helpful for guiding and improving ferroptosis-based anti-tumor therapy. To seek pro-ferroptosis regulators, we proposed a novel screening strategy which focuses on molecules consistently upregulated even after long-time treatment of ferroptosis inducer. RNA-seq of RSL3-treated cells was performed as to be analysed together with RNA-seq data of ferroptosis-resistant cells in GEO dataset. Cysteine-rich angiogenic inducer 61 (CYR61) was identified as a novel pro-ferroptosis regulator via our new mining strategy. The goal of this experiment was to characterize ferroptosis-related molecules. RNA-seq of H1299 cells treated with DMSO or RSL3 for 24 h in three replicates