Real-time quantitative PCR analysis of colon tissues of DSS-induced colitis mice.. Real-time quantitative PCR analysis of colon tissues of DSS-induced colitis mice.

Inflammatory bowel disease (IBD) is a multifactorial disease involving defective immune responses against invasive microbiota. A Rab32-dependent pathway that eliminates intracellular bacteria in dendritic cells (DCs) was recently reported. In this study, we examined whether Rab32 in DCs participates in the pathogenesis of IBD Overall design: WT and Rab32 dendritic cell specific knock out mice were administrated with 2.5% DSS for 9 days to induce colitis. On day 9, colon tissues were harvest for qPCR analysis. 3 mice in each group.