aUnderlined sequences are restriction enzymes. Bold sequence represents the histidine tag.* Negative PCR for B. ovis mutant strain.&Negative PCR for TT17573 strain.Primers for PCR used in this study.

Figshare2015-12-02 更新10

Additional file 10: of Expanding the biodiversity of Oenococcus oeni through comparative genomics of apple cider and kombucha strains

基因组学

PCR技术

Table S3. Primer list. The sequence surrounding NNN-islands in the CRBO_1381 assembly scaffold was entered into Primer3 with default settings (GC clamp = 1) to find suitable primersets for PCR product

Mendeley Data2024-06-27 更新70

Primers and probes for quantitation of mRNA by Q-PCR for rabbit BAFF, BR3, PPIA control and β2 microglobulin (B2M).

基因表达定量

PCR技术

aThe total volume of PCR reaction was 25 µl and the PCR conditions were as follows: 50°C for 2 min, 95°C for 10 min, followed by 40 cycles of 95°C, 15 s for denaturation and 60°C, 1 min for annealing

NIAID Data Ecosystem20