The point spread function of a fixed fluorophore with its dipole axis colinear to the optical axis appears donut-shaped when seen through a microscope, but can change to a spot when increasing the tub
Raw data used for creating Figure 5 of the paper "A Label-free Multicolor Optical Surface Tomography (ALMOST) imaging method for nontransparent 3D samples". Files are combined in compressed 7-Zip arch