Culture expansion of primary cells evokes highly reproducible DNA methylation (DNAm) changes at specific sites in the genome. These changes might be due to an directly regulated epigenetic process, or
Epidermis is the most outer layer of the skin and serves as a physical barrier protecting the internal tissues from mechanical and environmental insults. Its constant renewing and regeneration capacit
纯化的CD34+细胞通过造血干细胞扩增培养基培养7-8天后,在有或者无葡萄糖的条件下进行G-CSF、GM-CSF刺激后,使用TRIZOL法提取总RNA。通过Agilent Bioanalyzer 2100检测RNA质量后,进一步用RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#7925
Gene ontology terms for ASCs serially expanded in FBS and pHPL. Complete list of enriched GO terms for ASCs serially expanded in pHPL or FBS (P0 â P5). This data relates to Table 1. (XLSX 1017 kb)