MiaPaCa2 cells were treated (A) for 24 hr with 500 nM Torin-1 (* p < 0.0001), 10 nM rapamycin, or DMSO vehicle and (B) for 72 hr with 25 nM LP-4 (* p = 0.05) or non-targeting (nt) siRNA. Nuclei were
This is a FIJI macro that assists with quantifying nuclear size and shape, generating 3D distance maps of nuclei and locating locus tag puncta within the map thus producing a relative radial distance