chlange/streptococcus_thermophilus_fermentation_kaiser

--- license: cc-by-4.0 tags: - raman-spectroscopy - biotechnology - streptococcus-thermophilus - fermentation - batch-cultivation - process-analytical-technology - timegate pretty_name: Raman Spectra of Streptococcus thermophilus Fermentation Kaiser language: - en size_categories: - n<1K --- # Dataset Card for Raman Spectra from Fermentations of Streptococcus thermophilus Kaiser ## Dataset Summary This dataset contains offline Raman spectra collected during batch cultivations of ***Streptococcus thermophilus***. The data is intended for researchers developing chemometric models and machine learning pipelines for bioprocess monitoring. The dataset includes two distinct fermentation runs conducted in shake flasks over a 24-hour period, paired with reference analytical measurements (HPLC and spectrophotometry). --- ## Dataset Structure ### Data Points Each entry consists of a Raman spectrum (intensity vs. Raman shift) paired with the following quantitative annotations: * **Lactose [g/L]:** Primary carbon source. * **Galactose [g/L]:** Metabolic byproduct. * **Lactate [g/L]:** Primary fermentation product. * **OD600:** Optical density at 600 nm (biomass concentration). ### Preprocessing * **Status:** Raw. * **Note:** The spectra are provided in their raw format without baseline correction, smoothing, or normalization (e.g., no SNV or Savitzky-Golay filtering has been applied). --- ## Technical Specifications ### Cultivation Parameters * **Organism:** *Streptococcus thermophilus* * **Cultivation Mode:** Batch * **Vessel:** Shake flask * **Duration:** ~24 hours ### Measurement Setup * **Spectrometer:** Kaiser Raman RXN1 * **Sampling:** Offline samples taken throughout the fermentation. * **Reference Analytics:** * Metabolites measured via High-Performance Liquid Chromatography (HPLC). * Biomass measured via UV-Vis Spectrophotometry ($OD_{600}$). --- ## Dataset Sources - **Repository:** [https://huggingface.co/datasets/chlange/streptococcus_thermophilus_fermentation_kaiser](https://huggingface.co/datasets/chlange/streptococcus_thermophilus_fermentation_kaiser) - **Paper:** [Link to paper if available] ---许可协议：CC BY 4.0（知识共享署名4.0国际许可协议） tags: 标签：拉曼光谱（Raman Spectroscopy）、生物技术（Biotechnology）、嗜热链球菌（Streptococcus thermophilus）、发酵（Fermentation）、分批培养（Batch Cultivation）、过程分析技术（Process Analytical Technology）、Timegate pretty_name: 数据集名称：嗜热链球菌Kaiser菌株发酵拉曼光谱 language: 语言：英语 size_categories: 样本规模类别：少于1000条（n<1K） --- # 嗜热链球菌Kaiser菌株发酵拉曼光谱数据集卡片 ## 数据集概述 本数据集收录了嗜热链球菌（*Streptococcus thermophilus*）分批培养过程中采集的离线拉曼光谱，旨在为开发用于生物过程监测的化学计量学模型与机器学习流程的研究人员提供数据支持。 本数据集包含两组独立的摇瓶发酵实验，实验周期约24小时，同步配套了参考分析测量结果（高效液相色谱法与分光光度法）。 --- ## 数据集结构 ### 数据条目 每条数据均包含一条拉曼光谱（强度随拉曼位移变化）与以下定量注释信息： * **乳糖（Lactose, g/L）**：主要碳源。 * **半乳糖（Galactose, g/L）**：代谢副产物。 * **乳酸（Lactate, g/L）**：主要发酵产物。 * **光密度（OD₆₀₀）**：600 nm处的光密度，用于表征生物量浓度。 ### 预处理说明 * **预处理状态**：原始未处理 * **备注**：光谱以原始格式提供，未进行基线校正、平滑处理或归一化操作（例如未应用标准正态变量变换（Standard Normal Variate, SNV）或萨维茨基-戈莱（Savitzky-Golay）滤波）。 --- ## 技术规格 ### 培养参数 * **受试菌株**：嗜热链球菌（*Streptococcus thermophilus*） * **培养模式**：分批培养 * **培养容器**：摇瓶 * **培养周期**：约24小时 ### 测量设置 * **光谱仪**：凯泽（Kaiser）拉曼RXN1光谱仪 * **采样方式**：发酵过程中全程采集离线样品 * **参考分析方法**： * 代谢物通过高效液相色谱（High-Performance Liquid Chromatography, HPLC）测定 * 生物量通过紫外-可见分光光度法测定（$OD_{600}$） --- ## 数据集来源 - **数据集仓库**：[https://huggingface.co/datasets/chlange/streptococcus_thermophilus_fermentation_kaiser](https://huggingface.co/datasets/chlange/streptococcus_thermophilus_fermentation_kaiser) - **关联论文**：[如有则提供链接]