Analysis of independent transformants after pDblet + P<i>nmt1</i>-<i>EGFP</i> construction (Figure 3C).
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*1. The number corresponds to the number of isolates cloned in Figure 3C. *2. +: Plasmid is recovered from S. pombe in E. coli; −: Plasmid could not be recovered from S. pombe in E. coli. *3. Presence of desired insert is checked by restriction digestion. *4. The region between Pnmt1 and EGFP is sequenced using OHM025. *5. The insert is truncated.
创建时间:
2010-03-11



