Potential modulation of Puf3 chromatin association mediated by Rpb4

Gene expression involving RNA polymerase II is regulated by the concerted interplay between mRNA synthesis and degradation, a crosstalk where mRNA decay machinery impacts transcription and transcription machinery influences mRNA stability. Rpb4, and likely the dimer Rpb4/7 seem to be the central components of the RNA pol II governing these processes. Here we investigate the effect of ∆rrp4 on the potential chromatin association of the RNA binding protein Puf3 to chromatin in S. cerevisiae.涉及RNA聚合酶II（RNA polymerase II）的基因表达，受mRNA合成与降解的协同互作调控，二者构成交叉串扰：mRNA降解系统可影响转录过程，而转录系统亦可调控mRNA稳定性。Rpb4蛋白，以及大概率的Rpb4/7异二聚体，似乎是RNA聚合酶II中调控上述过程的核心组分。本研究探究了Δrrp4缺失突变体对酿酒酵母（S. cerevisiae）中RNA结合蛋白Puf3（RNA binding protein Puf3）染色质结合潜能的影响。