Gene expression levels of target genes were assessed by real time PCR in PBMCs from 16 new healthy controls after stimulation with IL10, IL4 or CTLA4 (stimulus) for 12 to 24h. Results are described as

Figshare2015-12-02 更新40

Quantitative PCR (RT-qPCR) validation of RNA-seq using liver samples from each of the four comparisons, illustrated in Fig 1.

基因表达验证

RNA-seq验证

Quantitative PCR (RT-qPCR) validation of RNA-seq using liver samples from each of the four comparisons, illustrated in Fig 1.

NIAID Data Ecosystem20

Validation of microarray data by using Real Time quantitative reverse transcriptase PCR analysis of representative genes from each functional category.

基因表达验证

RT-qPCR

aMean of two assays performed in duplicate.

NIAID Data Ecosystem50

Summary of differential expression false positives detected in non-native mapping cases.

转录组数据分析

RNA-seq假阳性检测

Summary of differential expression false positives detected in non-native mapping cases.

Figshare2017-07-12 更新30

RT-PCR validation of selected genes from microarray data.

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