Pranevicius et al_LAN_Raw Data

DAM2 Drosophila Activity Monitors (TriKinetics, Waltham, MA) were used to record the locomotor activity rhythms of adult male, and virgin female, flies aged three- to five-days, as previously described [39]. Flies were entrained to 12:12 LD or LAN cycles for at least five days and then transferred to constant darkness (DD) for at least eight days at a constant temperature of 25°C. Activity counts were collected in 1-minute intervals, which were subsequently binned into 30-minute intervals for conducting time-series analysis on locomotor activity. Averaged population activity profiles of specific genotypes in entrainment (LD or LAN) were generated in Matlab (MathWorks, Natick) using PHASE [40]. First, individual fly activity levels were normalized by establishing the average activity across all 30-minute bins over days 2-4 under LD or LAN as 1.0. Subsequently, population averages of this normalized activity were computed for each 30-minute bin. Finally, the population averages for the LD or LAN cycles were averaged into a single representative 24-hour day.