S. cerevisiae fragment datasets
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Results data of two-round SECRiFY screens of human (HEK293) cDNA fragments in S. cerevisiae (3 replicate screens). Sequencing data was mapped on the human GRCh38 transcriptome assembled using known transcripts from protein-coding genes only.<br>"Sc_resultstable_all.txt" = all in-frame fragments detected in either the unsorted baseline library (merged for 3 replicates), or in all 3 sorted replicate samples. <br>"Sc_resultstable_enriched.txt" = those with log_FC > 1 in all three replicates (11625)<br>"Sc_resultstable_depleted.txt" = those with log_FC < -1 in all three replicates (136531)<br><br>For each fragment, the following information is provided:<br><br># Ensembl_geneID --> Ensembl gene ID<br># Ensembl_txID --> Ensembl transcript ID<br># tx_start --> Transcript start position on human genome GRCh38<br># tx_end --> Transcript end position on human genome GRCh38<br># chr --> chromosome #<br># gene_symbol --> official gene symbol<br># frag_start --> fragment start position on the transcript, 0-based <br># frag_stop --> fragment end position on the transcript, 0-based<br># cDNA --> DNA sequence of the fragment<br># protein --> translated AA sequence of the fragment in frame 1<br># IND_count --> raw count value in the baseline (unsorted) library (merged for 3 replicates), NAs replaced by 0.001<br># SORT(1)_count --> raw count value in the sorted sample replicate (1), NAs replaced by 0.001<br># IND_FPTM --> normalized FPTM value in the baseline (unsorted) library <br># SORT(1)_FPTM --> normalized FPTM value in the sorted sample replicate (1)<br># logFC_(1) --> log2(SORT(1)_FPTM/IND_FPTM)<br>
