Real-time quantitative PCR analysis of human serum. Real-time quantitative PCR analysis of human serum

Blood samples for determination of serum miRNAs in acute myocardial infarction Overall design: qPCR miRNA expression profiling. 200 ul of Serum samples from 10 healthy individuals distributed in two pools of 5 individuals each and 3 patients per test group (STEMI/NSTEMI) were used for quantitative PCR Array screening of 752 miRNAs using the miRCURY LNA Universal RT microRNA PCR, 4x Human panel I+II in 384well PCR plates (type R), (Exiqon 203616).