Gene expression profiling reveals that ERα controls the mitochondrial biogenesis in LysM-Cre targeted cells. Gene expression profiling reveals that ERα controls the mitochondrial biogenesis in LysM-Cre targeted cells

Estrogens decrease osteoclast numbers via direct and indirect effects. In trabecular bone, the anti-osteoporotic efficacy of estrogens is mediated via the estrogen receptor α (ERα) present in myeloid lineage cells, but the molecular mechanism mediating these effects remain controversial. In contrast to published findings by others, FasLgld/gld mice which lack functional FasL lost cortical and cancellous bone following OVX indistinguishably from FasL-intact controls. Microarray analysis of osteoclast progenitors isolated from the bone marrow of ERαf/f;LysM-Cre mice and ERαf/f controls indicated that ERα deleted macrophages exhibited significant enrichment for the term “oxidative phosphorylation”, suggesting that estrogen signaling via ERα inhibits this process Overall design: Erα knock out is compare with control in osteoclast progenitors, pre-osteoclasts and mature osteoclasts