Comparison of the results of FMDV antigen detection methods using saliva of FMDV-inoculated pigs.

*MS: MSD-ELISA for multi-serotypes; SS: MSD-ELISA for single serotypes (O, A, Asia1); IS: Indirect sandwich-ELISA for each serotype (O, A, Asia1); rPCR: real-time RT-PCR.†The OD results (average sample OD-average buffer OD) of the MS, SS and IS ELISAs were as +++, >1.0; ++, 0.5–1.0; +, 0.1–0.5; and −, ‡The results-related plaque-forming unit of rPCR were as +++,>104; ++, 102–103; +, 100–102; and −, 0.§The pigs inoculated with virus were euthanized.∥Squares mean the day the obvious vesicular appeared except for the inoculated site.