Epigenome Mapping in pancreatic cancer cells
收藏NIAID Data Ecosystem2026-03-10 收录
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资源简介：
Genome binding/occupancy profiling of H3K4me1 and H3K4me3 upon KMT2D depletion was performed by Illumina NextSeq500 using single-end 75bp protocol. Overall design: Chromatin fragments, derived from siControl and siKMT2D (using 2 different siRNAs) treated cells, were immunoprecipitated with antibodies against Anti-Histone H3 (mono-methyl K4) and Anti-Histone H3 (tri-methyl K4). After purification of DNA from cell lysate by functionalized magnetic particles, libraries for next generation sequencing were prepared and analyzed using standard procedures.
创建时间：
2018-11-20
