Transcriptome analysis of cardiac specific depletion of RBPMS and RBPMS2 embryonic hearts [sch7_rnaseq]

To investigate the function of RNA-binding protein with multiple splicing (RBPMS) family, RBPMS and RBPMS2, in heart, we establish two RBPMS/2 double knock out mutant strains using cardiomyocyte specific Cre delete strains. Xenopus laevis light chain 2 (XMLC2) promoter CRE mice (Breckenridge et al., 2007) were crossed to RBPMSflox/flox / RBPMS2flox/flox animals to generate the RBPMS/2flox/flox / XMLC2-Cre mice line. In a parallel approach, we crossbred RBPMSflox/flox / RBPMS2flox/flox mice with animals carrying alpha myosin-heavy chain (Myh6) Cre (MyHC-Cre) (Agah et al., 1997), resulting in the RBPMS/2flox/flox / aMyHC-Cre line. RNA seq of E11.5 (XML-Cre) and E16.5 (MyHC-Cre) embryonic hearts was performed. Comparative gene expression profiling analysis of RNA-seq data for littermate control and cardiomyocyte specific double knock out of RBPMS/2 hearts at embryonic stage.为探究多剪接RNA结合蛋白家族（RNA-binding protein with multiple splicing, RBPMS）及其成员RBPMS、RBPMS2在心脏中的功能，我们利用心肌细胞特异性Cre重组酶敲除品系，构建了两种RBPMS/2双基因敲除突变小鼠品系。将非洲爪蟾肌球蛋白轻链2（Xenopus laevis light chain 2, XMLC2）启动子驱动的Cre重组酶小鼠（Breckenridge等，2007）与RBPMSflox/flox / RBPMS2flox/flox小鼠杂交，获得RBPMS/2flox/flox / XMLC2-Cre小鼠品系。在平行实验中，我们将RBPMSflox/flox / RBPMS2flox/flox小鼠与携带α肌球蛋白重链（alpha myosin-heavy chain, Myh6）Cre重组酶（MyHC-Cre）的小鼠杂交（Agah等，1997），得到RBPMS/2flox/flox / aMyHC-Cre品系。随后对E11.5（XML-Cre组）及E16.5（MyHC-Cre组）胚胎心脏开展RNA测序，并对同窝对照小鼠与胚胎时期心肌细胞特异性RBPMS/2双基因敲除小鼠的心脏RNA-seq数据进行比较基因表达谱分析。