The effect of stimulation by Gram-negative bacteria on the composition and biological activity of Lucilia sericata (Diptera: Calliphoridae)larval secretions– dataset

The dataset comprises: i) Chromatogram of induced <3 kDa L. sericata larval secretions (Figure 1). Chromatographic separation of induced and uninduced Ls larval S/E fractions (<3 kDa) was performed on a Superdex 30 Increase 10/300 GL column. Samples were eluted isocratically with deionised water (0.5 mL/min), and elution was monitored at 215 nm ii) Evaluation of larval secretion effects on HDMEC and HUVEC cell proliferation using the MTS assay Cells (Human Dermal Microvascular Endothelial Cells, HDMEC oraz Human Umbilical Vein Endothelial Cells, HUVEC) were seeded at 8×10³ cells per well in 100 µL complete medium. After 24 h, medium was replaced with serum- and supplement-free medium containing larval secretion fractions. Following 48 h incubation, cell proliferation was assessed using the MTS assay. Briefly, 20 µL MTS reagent and 100 µL fresh medium were added per well and incubated for 2 h at 37°C. Absorbance was measured at 490 nm using a microplate reader, and viability was expressed relative to untreated controls (100%). The results of the absorbance measurements in the MTS assay, as presented in the files (MTS_absorbance_HDMEC_raw data and descriptions_PA, MTS_absorbance_HUVEC_raw data and descriptions_PA) The work is supported by the National Science Centre, Poland: UMO-2021/43/D/NZ7/03119.