Adhesion of Marinobacter hydrocarbonoclasticus to surfactant-decorated oil droplets

This project investigated the effect of surfactant chemistry and concentration on the initial adhesion of Marinobacter hydrocarbonoclasticus at the interface of dodecane droplets suspended in water, investigating both the time-dependent adhesion of bacteria at the dodecane/water interface and the dependence of adhesion on droplet size and surfactant concentration. The dataset consists of images (.bmp) for dodecane-in-water emulsions prepared using co-flow microfluidic device, z-stack images (.tiff) for three different drop sizes at different time intervals, z-stack images (.tiff) for two different drop sizes at different surfactant concentrations and pendant drop movies (.avi files) at different surfactant concentrations for the dodecane/water system. The data analyzed consists of: (1) normalized drop diameter as a function of inner to outer fluid flow rates in microfluidic device, (2) number of cell and cells normalized with drop surface area as a function of time, (3) time constant of adhesion as a function of drop diameter, (4) orientation distribution of cells at dodecane/water interface for three drop sizes, (5) cell areal density as a function of surfactant concentration, (6) interfacial tension as a function of surfactant concentration, and (7) cell areal density as a function of interfacial tension.