Checklist for Comparison of feed-based dairy production of Pennisetum Sp, Pennisetum Purpureum and Ciat 36087

Checklist The ARRIVE Essential 10 Item Guidance for Reporting Reported in section Study design 1 For each experiment, provide brief details of study design including a.The groups being compared, including control groups. If no control group has been used, the rationale should be stated. Yes b.The experimental unit (e.g. a single animal, litter, or cage of animals) Yes Sample size 2 a. Specify the exact number of experimental units allocated to each group, and the total number in each experiment. Also indicate the total number of animals used. Yes b. Explain how the sample size was decided. Provide details of any a priori sample size calculation, if done Yes Inclusion and exclusion criteria 3 a. Describe any criteria used for including and excluding animals (or experimental units) during the experiment, and data points during the analysis. Specify if these criteria were established a priori. If no criteria were set, state this explicitly. Yes b. For each experimental group, report any animals, experimental units or data points not included in the analysis and explain why. If there were no exclusions, state so. Yes c. For each analysis, report the exact value of n in each experimental group Yes Randomisation 4 a. State whether randomisation was used to allocate experimental units to control and treatment groups. If done, provide the method used to generate the randomisation sequence. Yes b. Describe the strategy used to minimise potential confounders such as the order of treatments and measurements, or animal/cage location. If confounders were not controlled, state this explicitly Yes Blinding 5 Describe who was aware of the group allocation at the different stages of the experiment (during the allocation, the conduct of the experiment, the outcome assessment, and the data analysis). Yes Outcome measures 6 a. Clearly define all outcome measures assessed (e.g. cell death, molecular markers, or behavioural changes). Yes b. For hypothesis-testing studies, specify the primary outcome measure, i.e. the outcome measure that was used to determine the sample size. Yes Statistical methods 7 a. Provide details of the statistical methods used for each analysis, including software used. Yes b. Describe any methods used to assess whether the data met the assumptions of the statistical approach, and what was done if the assumptions were not me Experimental animals 8 a. Provide species-appropriate details of the animals used, including species, strain and substrain, sex, age or developmental stage, and, if relevant, weight. Yes b. Provide further relevant information on the provenance of animals, health/immune status, genetic modification status, genotype, and any previous procedures. Yes Experimental procedures 9 For each experimental group, including controls, describe the procedures in enough detail to allow others to replicate them, including: a. What was done, how it was done and what was used. Yes b. When and how often. Yes c. Where (including detail of any acclimatisation periods). Yes d. Why (provide rationale for procedures) Yes Results 10 For each experiment conducted, including independent replications, report: a. Summary/descriptive statistics for each experimental group, with a measure of variability where applicable (e.g. mean and SD, or median and range). Yes b. If applicable, the effect size with a confidence interval. Yes The Recommended Set Abstract 11 Provide an accurate summary of the research objectives, animal species, strain and sex, key methods, principal findings, and study conclusion Yes Background 12 a. Include sufficient scientific background to understand the rationale and context for the study, and explain the experimental approach. Yes b. Explain how the animal species and model used address the scientific objectives and, where appropriate, the relevance to human biology Yes Objectives 13 Clearly describe the research question, research objectives and, where appropriate, specific hypotheses being tested Yes Ethical statement 14 Provide the name of the ethical review committee or equivalent that has approved the use of animals in this study, and any relevant licence or protocol numbers (if applicable). If ethical approval was not sought or granted, provide a justification. Yes Housing and husbandry 15 Provide details of housing and husbandry conditions, including any environmental enrichment Yes Animal care and monitoring 16 a. Describe any interventions or steps taken in the experimental protocols to reduce pain, suffering and distress. Yes b. Report any expected or unexpected adverse events. Yes c. Describe the humane endpoints established for the study, the signs that were monitored and the frequency of monitoring. If the study did not have humane endpoints, state this. Yes Interpretation/ scientific implications 17 a. Interpret the results, taking into account the study objectives and hypotheses, current theory and other relevant studies in the literature. Yes b. Comment on the study limitations including potential sources of bias, limitations of the animal model, and imprecision associated with the results. Yes Generalisability/ translation 18 Comment on whether, and how, the findings of this study are likely to generalise to other species or experimental conditions, including any relevance to human biology (where appropriate Yes Protocol registration 19 Provide a statement indicating whether a protocol (including the research question, key design features, and analysis plan) was prepared before the study, and if and where this protocol was registered. Yes Data access 20 Provide a statement describing if and where study data are available. Yes Declaration of interests 21 a. Declare any potential conflicts of interest, including financial and non-financial. If none exist, this should be stated. Yes b. List all funding sources (including grant identifier) and the role of the funder(s) in the design, analysis and reporting of the study Yes Data The data describes the process and the management of the information for the study that compares the milk production and the production costs of a liter of milk of 33 dairy cows of two breeds: Brown Swiss and Jersey, fed with Maralfalfa (Pennisetun Sp) pastures. , Cameroon (Pennisetum purpureum) and Mulatto (Ciat 36087). The productive and reproductive biological variables with an impact on milk production are analyzed, including the most important days open, age at first calving, number of calvings and live weight. The basic selection criteria of the breeders for our study was that they had less than 60 open days; Selected cows should be in peak lactation. The milk production of the cows under study fed only with grazing Mulato was evaluated for 30 days, to generate a productive record and to have a productive history of these. After 30 days of monitoring milk production, the group of 33 cows was randomly divided into 3 groups, 11 cows per group, and they were assigned the type of grass to consume, establishing the Maralfalfa consumption group (G1). the consumer group from Cameroon (G2) and the control group with grazing mulatto (G3). A record of daily milk production of each cow was kept for 6 uninterrupted weeks, they were given equal management, in the same environment and with ad libitum feeding to the 3 groups under study with the only difference being the type of grass to be consumed. . When comparing the milk production of the groups of cows under study using the 1-Factor Anova statistical program, we found very significant statistical differences (p < 0.01) between the three groups studied from week 2 onwards. The most marked difference was presented by the G1 vs. G3 group. According to our results, the grass with the best productive efficiency for milk production is the Maralfalfa grass, since the group that consumed this grass was the one that presented the greatest increases in production volumes. However, when comparing the production costs of a liter of milk, we find that the highest cost is for maralfalfa and the lowest for mulatto and therefore the cost-benefit ratio is in favor of mulatto followed by Cameroon and maralfalfa respectively.