Challenges with using Primer IDs to improve accuracy of next generation sequencing. Primer IDs in next-generation sequencing

The dataset was used to study the challenges with using Primer IDs in next generation sequencing data. Primer ID was applied on a ultra deep pyrosequencing reads generated from a HIV-1 SG3∆env plasmid clone and plasma samples from three HIV-infected patients. The Primer ID consisted of 11 randomized nucleotides.