COS7 CHMP4B+VPS4DN

Tilt Series Date: 2015-02-07 Data Taken By: Cora Woodward Species / Specimen: Cercopithecus aethiops (monkey, African green) Strain: COS-7 ATCC CRL-1651 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward Purification / Growth Conditions / Treatment: whole cells were grown on grids and transfected with Lipofectamine Sample Preparation: blotting倾斜系列采集日期：2015-02-07；数据采集者：科拉·伍德沃德。物种/标本：非洲绿猴（Cercopithecus aethiops，African green monkey）。菌株：COS-7 美国典型培养物保藏中心（ATCC）CRL-1651。倾斜系列设置：单轴倾斜模式，倾斜范围为(-60.0°, 60.0°)，步长1.0°，采用恒定角度增量，辐照剂量为150.0 eV/Ų，散焦值为-6.0 μm，放大倍数22500倍。采集软件：UCSFTomo。上传方式：流水线上传。所用图像处理软件：Raptor。合作者及分工：载体构建由韦斯·桑德奎斯特（Wes Sundquist）提供；细胞转染、冷冻及成像实验由科拉·伍德沃德于加州理工学院（Caltech）完成。纯化/培养条件/处理方式：全细胞培养于载网之上，并用脂质体转染试剂（Lipofectamine）进行转染。样品制备：印迹法（blotting）。