FIG 5 Nickel enhancement of HOSCN toxicity is independent of RclABC. E. coli strains MG1655 (wild-type), MJG1958 (MG1655 ∆ rclA747 ), and MJG0901 (MG1655 ∆ rclABC1 ) were grown
FIG S6 Ability of transition metals (Cu, Zn, Ni) to oxidize TNB. Oxidation of 30 µM 2-nitro-5-thiobenzoic acid (TNB) in 10 mM HEPES-KOH (pH 7.4) by addition of 1 µM CuCl2, ZnSO4, or NiSO4 was measured
FIG S3 Deletions of copper and nickel exporters have small effects on E. coli sensitivity to HOSCN in the presence of their respective metals. E. coli strains MG1655
FIG 8 Glutathione reductase is inhibited by nickel but not by HOSCN. Glutathione and thioredoxin reductase activity was measured in cell-free lysates of E. coli MG1655. Glutathione reductase assays (A
