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DNA oligos used for Q-PCR.
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2016-04-14
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Additional file 10: of Expanding the biodiversity of Oenococcus oeni through comparative genomics of apple cider and kombucha strains
Table S3. Primer list. The sequence surrounding NNN-islands in the CRBO_1381 assembly scaffold was entered into Primer3 with default settings (GC clamp = 1) to find suitable primersets for PCR product
Mendeley Data2024-06-27 更新70
Sequence of the primers used in the experiment.
Sequence of the primers used in the experiment.
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The primer sequences used in the RT-PCR.
The primer sequences used in the RT-PCR.
Figshare2017-07-28 更新20
Characteristics of the primers used for SYBR green Q-PCR assays.
*Letters F and R after the primer name indicate forward and reverse orientation, respectively. †Theoretical melting temperature (Tm) calculated using the MWG Oligo Property Scan (MOPS).
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Primers and probes for quantitation of mRNA by Q-PCR for rabbit BAFF, BR3, PPIA control and β2 microglobulin (B2M).
aThe total volume of PCR reaction was 25 µl and the PCR conditions were as follows: 50°C for 2 min, 95°C for 10 min, followed by 40 cycles of 95°C, 15 s for denaturation and 60°C, 1 min for annealing
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