Subcellular fractionation of transfected HEK293 cells.
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HEK293 cells stably expressing VWF-CFP were transfected with FVIII-YFP variants. FVIII antigen was quantified by anti-FVIII light chain ELISA. VWF antigen was quantified by ELISA. The amount of FVIII and VWF antigen in the pooled fractions 4–9 is divided by the total amount of FVIII and VWF antigen in the pooled fractions 1–25. The values represent the mean ± SD of three measurements.
创建时间:
2011-08-31



