Mesenchymal Stem Cells (MSCs) Alleviate Experimental Immune-mediated Liver Injury via Chitinase 3-like protein 1-mediated T cell suppression

RNA-seq for MSCs from human bone marrow (BM-MSCs), olfactory mucosa (OM-MSCs), and umbilical cord (UC-MSCs) (2 donors for each). RNA-sequencing for human naïve CD3+ T cells, CD3+ T cells activated with PHA (2.5 µg/mL) for 48 h, and CD3+ T cells co-cultured with human UC-MSCs in the presence of PHA for 48 h. Overall design: MSCs from bone marrow (BM-MSCs), olfactory mucosa (OM-MSCs), and umbilical cord (UC-MSCs) (2 donors for each) were collected and sequenced to identify the differently expressed genes and related pathways. FACS sorted human naïve CD3+ T cells, CD3+ T cells activated with PHA (2.5 µg/mL) for 48 h, and CD3+ T cells co-cultured with human UC-MSCs in the presence of PHA for 48 h were collected and sequenced to identify the differently expressed genes and related pathways.