scRNA and TCR seq of CD8 TILs of WT and Card11 mutant mice(E134G/K215M)

To investigate the impact of Card11 on TIL differentiation, we isolated TILs from B16-F10 tumors of WT mice, E134G mice, and K215M mice and performed scRNA seq. We also sorted tumor-infiltrating lymphocytes (TILs) from melanoma(B16-F10) of WT mice and conducted scRNA and scTCR sequencing. To further investigate the impact of Card11 on TCR clonal diversity, we sorted CD8 T cells from melanoma WT mice or K215M mice and performed scRNA and scTCR sequencing. scRNA and TCR seq of CD8 TILs of WT and Card11 mutant mice(E134G/K215M)