Comparison of MPA regulated gene expression profiles to those regulated by PROG, DHT, DEX

Medroxyprogesterone acetate (MPA) is a progestin that can bind to and activate progesterone, androgen and glucocorticoid receptors. However, it is not known which receptor mediates MPA action in a cellular context where all three of these receptors are co-expressed and functional. This microarray experiment was performed to compare the transcriptomes induced by MPA and the cognate ligands for these receptors ie progesterone (PROG), 5a-dihydrotestosterone (DHT) and dexamethasone (DEX) in breast cancer cells to determine which was most similar to MPA. Transcriptome analysis by microarray for ZR-75-1 breast cancer cells treated for 6 hours with 10 nM MPA, PROG, DHT, DEX or vehicle.