Expression data from mouse brain mononuclear cells. Expression data from mouse brain mononuclear cells

A membrane protein SIRPα (CD172a) interacts with another membrane protein CD47 and thereby constitutes a cell–cell contact signal. To investigate the functional role of CD47-SIRPα signal in the brain, we analyzed the effect of genetic ablation of CD47-SIRPα signal on the gene expression profile in specific brain cells by the use of microarrays. Overall design: Total RNAs were prepared from the brain mononuclear cells of wild-type or CD47 knockout mice. Brain mononuclear cells were isolated by the use of Percoll gradient method. Total RNAs from seven (wild-type) and six (CD47 knockout) different animals were pooled and subjected to the analysis on Affymetrix Gene Chip.