C6 glioblastoma microRNA regulation induced by haloperidol, risperidone and clozapine

Antipsychotic drugs (APDs) have been reported to ease psychotic symptoms in the clinic. miRNAs have also been identified that can regulate the expression of genes/proteins related to possible molecular mechanisms of drug action. To reveal APD-induced miRNA modulations in glial cells, we analyzed miRNA expression in subchronically APD-treated C6 cells by using a miRNA microarray. C6 cells were subchronically treated with haloperidol, risperidone or clozapine for five days. The final concentrations of haloperidol (2 μg/ml), risperidone (4 μg/ml) and clozapine (25 μg/ml) were calculated based on therapeutic concentrations in clinic. Cells were then harvested for microarray analysis. miRNA expression of APD-treated C6 cells were compared to miRNA expression of untreated control C6 cells. The microarray experiments were performed in two biological repeats.