Transcriptome reprogramming via Tob2-promoted global mRNA turnover

We found that multilayered phosphorylation of human Tob2 modulates its PABP-binding ability to change global deadenylation and mRNA turnover, which in turn reprograms transcriptome to influence cell proliferation. Overall design: Bromouridine-metabolic labeling pulse-chase RIP-seq approach was combined with time-course experiments to monitor mRNA decay across the transcriptome upon induced expression of a phosphomimetic mutant (S254D) of Tob2.