Next Generation sequencing and quantitative transcriptomic analysis in -77+/+ and -77-/- erythroid precursors

Gene expression profiles of sorted erythroid precursors from E14.5 wild-type (WT) and -77 enhancer knockout (-77-/-) mouse fetal liver were generated by next generation sequencing, in triplicate, using Illumina Hiseq2500 (1X50). We showed that the Gata2 -77 enhancer generated the vital erythroid precursor Burst Forming Unit-Erythroid by two mechanisms, GATA-1 upregulation to establish the erythroid transcriptome and GATA-2-dependent, GATA-1-independent transcriptional regulation. Erythroid precursor gene expression profiles of E14.5 mouse -77+/+ and -77-/- mouse fetal livers were generated by next generation sequencing, in triplicate, using Illumina Hiseq2500 (1X50).本数据集包含取自胚胎发育第14.5天（E14.5）野生型（wild-type, WT）及-77增强子敲除（-77 enhancer knockout, -77-/-）小鼠胎肝的分选红细胞前体细胞的基因表达谱，采用二代测序（next generation sequencing）技术，借助Illumina Hiseq2500（1X50）平台完成检测，设置三次生物学重复。本研究证实，Gata2基因的-77增强子可通过两种机制调控关键红细胞前体细胞——爆式集落形成单位-红细胞（Burst Forming Unit-Erythroid, BFU-E）的生成：其一为上调GATA-1以构建红细胞特异性转录组；其二为GATA-2依赖且GATA-1非依赖的转录调控通路。本研究同时通过二代测序技术，借助Illumina Hiseq2500（1X50）平台完成三次生物学重复测序，获取了E14.5天-77+/+及-77-/-小鼠胎肝的红细胞前体细胞基因表达谱。