Bone (femur) gene expression (mRNA) as measured by quantitative RT/PCR (qRT-PCR) for wild type and HYP mice infused with vehicle or SPR4-peptide (see also Figure 4).

Column headings represent; WT = wild type mice, HYP = X-linked hypophosphatemic rickets mice, SPR4 = infused SPR4-peptide and Vehicle = Saline infused. For gene analysis mice were sacrificed on day 28 and mRNA prepared from bone marrow stromal cell depleted femurs as detailed in methods (N = 6). For qRT-PCR gene analysis fold differences in expression calculated by the Pfaffl method [163] were statistically analyzed for significance using the One Sample t-test and the Wilcoxon Signed rank-test with theoretical means set to 1. Results for all tests were considered to be significantly different at pWT-VE versus HYP-VE, WT-VE versus WT-SPR4 and HYP-VE versus HYP-SPR4 are shown in columns 1, 2 and 3 as indicated in the headings. ND = Not done, NS = Not Significant. Index:FGF23 = Fibroblast Growth Factor 23; MEPE = Matrix Extracellular Phosphoglycoprotein with ASARM -motif; DMP1 = Dentin Matrix Protein 1; SOST = Sclerostin; VEGF = Vascular Endothelial Growth factor; GAPDH = Glyceraldehyde 3-phosphate dehydrogenase; PHEX = Phosphate-regulating gene with Homologies to Endopeptidases on the X chromosome; Osteocalcin = Bone gamma-carboxyglutamate (gla) protein; Cyclophilin = peptidylprolyl isomerase A (cyclophilin A); Plin-2 = Perlipin-2; ESP = Osteotesticular protein tyrosine phosphatase (OST-PTP); FAM20C = Family with sequence similarity 20, member C Kinase also known as DMP4; ENPP1 = Ectonucleotide Pyrophosphatase Phosphodiesterase 1.