Additional file 2: of ACTL6A expression promotes invasion, metastasis and epithelial mesenchymal transition of colon cancer

Figure S1. (A) The ACTL6A interfered efficiency was assessed by real-time PCR, results showed that either overexpressed or knocked down plasmid had satisfied interfered efficiency and RNAi-1 had the strongest knockdown efficiency. (B) MTT assay showed that ACTL6A overexpression didn’t significant increased the proliferation rate of SW480 cells (P = 0.099), while ACTL6A knockdown slightly decreased the proliferation rate of SW620 cells (P < 0.05). (C) Plate colony formation assay showed that ACTL6A overexpression mildly increased the colony numbers of SW480 cells (P = 0.041), while ACTL6A knockdown had no significant effect in proliferation of SW620 cells (P = 0.074). (ZIP 256 kb)