Rfam

The Rfam database, a widely used repository of non-coding RNA families, has undergone significant updates in release 15.0. This paper introduces major improvements, including the expansion of Rfamseq to 26 106 genomes, a 76% increase, incorporating the latest UniProt reference proteomes and additional viral genomes. Sixty-five RNA families were enhanced using experimentally determined 3D structures, improving the accuracy of consensus secondary structures and annotations. R-scape covariation analysis was used to refine structural predictions in 26 families. Gene Ontology (GO) and Sequence Ontology annotations were comprehensively updated, increasing GO term coverage to 75% of families.作为一款广泛使用的非编码RNA（non-coding RNA）家族数据库，Rfam数据库在15.0版本中完成了重大更新。本文详细介绍了该版本的主要改进内容：其一，Rfamseq序列库扩增至26106个基因组，增幅达76%，并纳入了最新的UniProt参考蛋白质组与新增的病毒基因组；其二，65个RNA家族通过实验测定的三维结构得到优化，提升了共识二级结构与注释信息的准确性；其三，研究团队使用R-scape协变分析工具对26个家族的结构预测结果进行了细化优化；其四，基因本体（Gene Ontology，GO）与序列本体（Sequence Ontology）注释信息得到全面更新，使得GO术语的覆盖范围提升至所有家族的75%。

搜集汇总

背景与挑战

背景概述

Rfam是一个广泛使用的非编码RNA家族数据库，包含多个序列比对、共识二级结构和协方差模型。在15.0版本中，它显著扩展了基因组覆盖范围，并利用实验性3D结构增强了多个家族的准确性和注释。