Dysbiotic oral microbiota contributes to alveolar bone loss associated with obesity in mice

The current study aimed to relate obesity, changes in oral microbiota, and induction of alveolar bone loss, as seen in PD. Two obesity mice models were performed, one using 12 weeks of the dietary protocol with a high-fat (HF) diet in C57BL/6 mice and the other using leptin receptor-deficient mice (db/db-/-), which became spontaneously obese. After euthanasia, the alveolar bone loss was evaluated by microCT, and the microbiota composition of mice fed or not with the HF diet was assessed by DNA-DNA hybridization technique. Topical application of chlorhexidine (CHX) was used to investigate the impact of the oral microbiota on the alveolar bone loss associated with obesity.