Small RNA-seq of Ago1 and Ago1x immunoprecipitated samples

Purpose: To identify small RNAs interacting with Ago1x Methods: FLAG-HA tagged Ago proteins were immunoprecipitated and associated miRNAs were isolated using miRNeasy Mini kit (Qiagen) Results: Interaction of Ago1x with miRNAs is comparable to that Ago1 Overall design: Small RNA seq was done for RNA isolated from immunoprecipitated samples