DYRK2 knockout mice exhibit fetal growth retardation

We generated DYRK2-deficient mice using the CRISPR/Cas9 genome editing method and demonstrated that loss of DYRK2 gene causes fetal growth retardation and neonatal lethality at birth. Total RNA from DYRK2-/- whole embryo was compared with those of WT mice by microarray. mRNA expression of whole embryo from DYRK2 knockout mice was compared with those of WT mice.本研究通过CRISPR/Cas9基因组编辑技术构建了DYRK2基因缺陷型小鼠模型，并证实DYRK2基因缺失会引发胎儿生长迟缓以及新生小鼠出生时致死的表型。采用基因芯片（microarray）技术，对DYRK2纯合敲除（DYRK2-/-）小鼠全胚胎的总RNA与野生型（Wild Type, WT）小鼠的总RNA进行了表达差异对比分析。此外，本研究还针对DYRK2敲除小鼠全胚胎的mRNA表达谱与野生型小鼠的mRNA表达谱开展了比对分析。