The relationship between the qPCR cycle threshold (Ct) values and the subsequent success of sequencing.
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Four specimens with Ct values between 20 and 28 generated complete DENV-3 by Shotgun metagenomics. Three specimens with Ct values between 27 and 32 generated partial DENV-3 genomes but with the complete E gene. Ten specimens with Ct values between 25 and 36 generated partial DENV-3 genomes with incomplete E gene. Complete E gene was obtained after targeted sequencing of these 10 samples. Four samples with Ct values >36 failed to produce E gene on targeted sequencing.
创建时间:
2022-03-24



